从尝试运行 ggplot（） 时收到“错误：必须从色调调色板请求至少一种颜色”

问：

遇到一些错误导致我无法绘制 PCA 图？这是我在终端上运行这组代码时的输出。

> #pdf(file="pca.mouse.control.pdf")
> #performing PCA using top 500 varying genes
> pcaData<-plotPCA(logTrans, intgroup=c("condition","time"), ntop=3000,returnData=TRUE) #top 1000
> percentVar<-round(100*attr(pcaData,"percentVar")) #percent variance caused by pca1, pca2 rounded to a percentage
> #coloring the points by Condition and shaping the points by Sex
> ggplot(pcaData, aes(PC1, PC2, color=condition, shape=time))+
+   geom_point(size=3)+
+   xlab(paste0("PC1: ",percentVar[1],
+               "%variance"))+
+   ylab(paste0("PC2: ",percentVar[2],
+               "%variance"))+
+   coord_fixed()
Error: Must request at least one colour from a hue palette.

我似乎没有添加颜色或要求调色板？我很困惑这个错误是从哪里来的。

我尝试用括号进行修改，但它们对我根本没有帮助。也许是括号，但我目前没有把它们弄对。需要一些帮助。

编辑： logTrans 上下文

> dput(sampleTable)
structure(list(trash = c("024_S113.gff", "067_S146.gff", "076_S154.gff", 
"084_S160.gff", "093_S168.gff", "104_S178.gff", "111_S185.gff", 
"012_S103.gff", "029_S118.gff", "050_S134.gff", "052_S135.gff", 
"083_S159.gff", "086_S162.gff", "005_S99.gff", "013_S104.gff", 
"057_S139.gff", "061_S142.gff", "064_S144.gff", "071_S149.gff", 
"098_S172.gff", "117_S190.gff", "014_S105.gff", "023_S112.gff", 
"027_S116.gff", "037_S125.gff", "045_S131.gff", "046_S132.gff", 
"088_S164.gff", "090_S166.gff", "070_S148.gff", "081_S157.gff", 
"082_S158.gff", "087_S163.gff", "105_S179.gff", "010_S102.gff", 
"020_S109.gff", "021_S110.gff", "034_S122.gff", "036_S124.gff", 
"068_S147.gff", "073_S151.gff", "074_S152.gff", "110_S184.gff", 
"022_S111.gff", "031_S120.gff", "035_S123.gff", "049_S133.gff", 
"072_S150.gff", "085_S161.gff", "089_S165.gff", "099_S173.gff", 
"101_S175.gff", "015_S106.gff", "026_S115.gff", "053_S136.gff", 
"065_S145.gff", "096_S170.gff", "109_S183.gff", "118_S191.gff", 
"001_S96.gff", "004_S98.gff", "007_S100.gff", "017_S107.gff", 
"028_S117.gff", "059_S141.gff", "078_S155.gff", "103_S177.gff", 
"106_S180.gff", "115_S188.gff", "002_S97.gff", "041_S128.gff", 
"063_S143.gff", "075_S153.gff", "080_S156.gff", "095_S169.gff", 
"102_S176.gff", "107_S181.gff", "114_S187.gff", "008_S101.gff", 
"019_S108.gff", "032_S121.gff", "038_S126.gff", "040_S127.gff", 
"091_S167.gff", "097_S171.gff", "100_S174.gff", "025_S114.gff", 
"030_S119.gff", "042_S129.gff", "044_S130.gff", "055_S137.gff", 
"056_S138.gff", "058_S140.gff", "108_S182.gff", "113_S186.gff", 
"116_S189.gff", "119_S192.gff", "121_S193.gff", "122_S194.gff", 
"124_S195.gff", "125_S196.gff", "126_S197.gff", "127_S198.gff", 
"128_S199.gff", "129_S200.gff", "130_S201.gff", "131_S202.gff", 
"132_S203.gff", "039_S211.gff", "043_S212.gff", "120_S213.gff", 
"123_S214.gff", "018_S204.gff", "033_S205.gff", "051_S206.gff", 
"062_S207.gff", "066_S208.gff", "077_S209.gff", "094_S210.gff"
), filename = c("A-000h-024_S113.gff", "A-000h-067_S146.gff", 
"A-000h-076_S154.gff", "A-000h-084_S160.gff", "A-000h-093_S168.gff", 
"A-000h-104_S178.gff", "A-000h-111_S185.gff", "A-072h-012_S103.gff", 
"A-072h-029_S118.gff", "A-072h-050_S134.gff", "A-072h-052_S135.gff", 
"A-072h-083_S159.gff", "A-072h-086_S162.gff", "A-168h-005_S99.gff", 
"A-168h-013_S104.gff", "A-168h-057_S139.gff", "A-168h-061_S142.gff", 
"A-168h-064_S144.gff", "A-168h-071_S149.gff", "A-168h-098_S172.gff", 
"A-168h-117_S190.gff", "B-000h-014_S105.gff", "B-000h-023_S112.gff", 
"B-000h-027_S116.gff", "B-000h-037_S125.gff", "B-000h-045_S131.gff", 
"B-000h-046_S132.gff", "B-000h-088_S164.gff", "B-000h-090_S166.gff", 
"B-072h-070_S148.gff", "B-072h-081_S157.gff", "B-072h-082_S158.gff", 
"B-072h-087_S163.gff", "B-072h-105_S179.gff", "B-168h-010_S102.gff", 
"B-168h-020_S109.gff", "B-168h-021_S110.gff", "B-168h-034_S122.gff", 
"B-168h-036_S124.gff", "B-168h-068_S147.gff", "B-168h-073_S151.gff", 
"B-168h-074_S152.gff", "B-168h-110_S184.gff", "C-000h-022_S111.gff", 
"C-000h-031_S120.gff", "C-000h-035_S123.gff", "C-000h-049_S133.gff", 
"C-000h-072_S150.gff", "C-000h-085_S161.gff", "C-000h-089_S165.gff", 
"C-000h-099_S173.gff", "C-000h-101_S175.gff", "C-072h-015_S106.gff", 
"C-072h-026_S115.gff", "C-072h-053_S136.gff", "C-072h-065_S145.gff", 
"C-072h-096_S170.gff", "C-072h-109_S183.gff", "C-072h-118_S191.gff", 
"C-168h-001_S96.gff", "C-168h-004_S98.gff", "C-168h-007_S100.gff", 
"C-168h-017_S107.gff", "C-168h-028_S117.gff", "C-168h-059_S141.gff", 
"C-168h-078_S155.gff", "C-168h-103_S177.gff", "C-168h-106_S180.gff", 
"C-168h-115_S188.gff", "D-000h-002_S97.gff", "D-000h-041_S128.gff", 
"D-000h-063_S143.gff", "D-000h-075_S153.gff", "D-000h-080_S156.gff", 
"D-000h-095_S169.gff", "D-000h-102_S176.gff", "D-000h-107_S181.gff", 
"D-000h-114_S187.gff", "D-072h-008_S101.gff", "D-072h-019_S108.gff", 
"D-072h-032_S121.gff", "D-072h-038_S126.gff", "D-072h-040_S127.gff", 
"D-072h-091_S167.gff", "D-072h-097_S171.gff", "D-072h-100_S174.gff", 
"D-168h-025_S114.gff", "D-168h-030_S119.gff", "D-168h-042_S129.gff", 
"D-168h-044_S130.gff", "D-168h-055_S137.gff", "D-168h-056_S138.gff", 
"D-168h-058_S140.gff", "D-168h-108_S182.gff", "D-168h-113_S186.gff", 
"D-168h-116_S189.gff", "N-000h-119_S192.gff", "N-000h-121_S193.gff", 
"N-000h-122_S194.gff", "N-000h-124_S195.gff", "N-000h-125_S196.gff", 
"N-000h-126_S197.gff", "N-000h-127_S198.gff", "N-000h-128_S199.gff", 
"N-000h-129_S200.gff", "N-000h-130_S201.gff", "N-000h-131_S202.gff", 
"N-000h-132_S203.gff", "X-000h-039_S211.gff", "X-000h-043_S212.gff", 
"X-000h-120_S213.gff", "X-000h-123_S214.gff", "X-072h-018_S204.gff", 
"X-072h-033_S205.gff", "X-072h-051_S206.gff", "X-072h-062_S207.gff", 
"X-072h-066_S208.gff", "X-072h-077_S209.gff", "X-072h-094_S210.gff"
), treatment_group = c("A", "A", "A", "A", "A", "A", "A", "A", 
"A", "A", "A", "A", "A", "A", "A", "A", "A", "A", "A", "A", "A", 
"B", "B", "B", "B", "B", "B", "B", "B", "B", "B", "B", "B", "B", 
"B", "B", "B", "B", "B", "B", "B", "B", "B", "C", "C", "C", "C", 
"C", "C", "C", "C", "C", "C", "C", "C", "C", "C", "C", "C", "C", 
"C", "C", "C", "C", "C", "C", "C", "C", "C", "D", "D", "D", "D", 
"D", "D", "D", "D", "D", "D", "D", "D", "D", "D", "D", "D", "D", 
"D", "D", "D", "D", "D", "D", "D", "D", "D", "D", "N", "N", "N", 
"N", "N", "N", "N", "N", "N", "N", "N", "N", "X", "X", "X", "X", 
"X", "X", "X", "X", "X", "X", "X"), time = c("000h", "000h", 
"000h", "000h", "000h", "000h", "000h", "072h", "072h", "072h", 
"072h", "072h", "072h", "168h", "168h", "168h", "168h", "168h", 
"168h", "168h", "168h", "000h", "000h", "000h", "000h", "000h", 
"000h", "000h", "000h", "072h", "072h", "072h", "072h", "072h", 
"168h", "168h", "168h", "168h", "168h", "168h", "168h", "168h", 
"168h", "000h", "000h", "000h", "000h", "000h", "000h", "000h", 
"000h", "000h", "072h", "072h", "072h", "072h", "072h", "072h", 
"072h", "168h", "168h", "168h", "168h", "168h", "168h", "168h", 
"168h", "168h", "168h", "000h", "000h", "000h", "000h", "000h", 
"000h", "000h", "000h", "000h", "072h", "072h", "072h", "072h", 
"072h", "072h", "072h", "072h", "168h", "168h", "168h", "168h", 
"168h", "168h", "168h", "168h", "168h", "168h", "000h", "000h", 
"000h", "000h", "000h", "000h", "000h", "000h", "000h", "000h", 
"000h", "000h", "000h", "000h", "000h", "000h", "072h", "072h", 
"072h", "072h", "072h", "072h", "072h")), row.names = c(NA, -119L
), class = c("tbl_df", "tbl", "data.frame"))



    ddsHTSeq<-DESeqDataSetFromHTSeqCount(sampleTable=sampleTable, directory=directory, design=~treatment_group+time) 
    ddsHTSeq<- estimateSizeFactors(ddsHTSeq)
    logTrans<- vst(ddsHTSeq) #normalized counts

dput（pca数据）

> pcaData
                          PC1        PC2   group condition time            name
A-000h-024_S113  -0.320694178  2.7778412 NA:000h      <NA> 000h A-000h-024_S113
A-000h-067_S146  -0.002410985  8.3898396 NA:000h      <NA> 000h A-000h-067_S146
A-000h-076_S154   0.499607961  3.7851280 NA:000h      <NA> 000h A-000h-076_S154
A-000h-084_S160   2.067338462  3.3101123 NA:000h      <NA> 000h A-000h-084_S160
A-000h-093_S168   3.111691815  1.7002517 NA:000h      <NA> 000h A-000h-093_S168
A-000h-104_S178  -3.818532232  1.1508051 NA:000h      <NA> 000h A-000h-104_S178
A-000h-111_S185  -3.742749898  3.5110641 NA:000h      <NA> 000h A-000h-111_S185
A-072h-012_S103  -0.534166142  1.8638765 NA:072h      <NA> 072h A-072h-012_S103
A-072h-029_S118  -0.836948039  0.2941960 NA:072h      <NA> 072h A-072h-029_S118
A-072h-050_S134   2.960265751 -0.6157736 NA:072h      <NA> 072h A-072h-050_S134
A-072h-052_S135   3.525457747 -2.4132327 NA:072h      <NA> 072h A-072h-052_S135
A-072h-083_S159   2.409759291 -0.1038610 NA:072h      <NA> 072h A-072h-083_S159
A-072h-086_S162   3.722458157  5.0023295 NA:072h      <NA> 072h A-072h-086_S162
A-168h-005_S99    2.146962939 -0.0394367 NA:168h      <NA> 168h  A-168h-005_S99
A-168h-013_S104   2.460944560 -2.6037950 NA:168h      <NA> 168h A-168h-013_S104
A-168h-057_S139   0.726681360  0.3563937 NA:168h      <NA> 168h A-168h-057_S139
A-168h-061_S142   3.054806788 -2.7197222 NA:168h      <NA> 168h A-168h-061_S142
A-168h-064_S144   2.848271362 -1.8982479 NA:168h      <NA> 168h A-168h-064_S144
A-168h-071_S149   1.305806698 -2.6754773 NA:168h      <NA> 168h A-168h-071_S149
A-168h-098_S172   2.567909657 -0.8122940 NA:168h      <NA> 168h A-168h-098_S172
A-168h-117_S190  -8.141619722 -2.5115206 NA:168h      <NA> 168h A-168h-117_S190
B-000h-014_S105   2.370623844  2.5494356 NA:000h      <NA> 000h B-000h-014_S105
B-000h-023_S112   0.658832442  3.3414638 NA:000h      <NA> 000h B-000h-023_S112
B-000h-027_S116  -0.869408244  2.7761878 NA:000h      <NA> 000h B-000h-027_S116
B-000h-037_S125   6.529922314  1.8519230 NA:000h      <NA> 000h B-000h-037_S125
B-000h-045_S131   3.880650507  4.0539278 NA:000h      <NA> 000h B-000h-045_S131
B-000h-046_S132   2.862712194  3.9337226 NA:000h      <NA> 000h B-000h-046_S132
B-000h-088_S164   1.847039948  6.1028658 NA:000h      <NA> 000h B-000h-088_S164
B-000h-090_S166   0.849799101  2.5122905 NA:000h      <NA> 000h B-000h-090_S166
B-072h-070_S148   0.359739401 -1.7607710 NA:072h      <NA> 072h B-072h-070_S148
B-072h-081_S157   1.099741212 -0.5528003 NA:072h      <NA> 072h B-072h-081_S157
B-072h-082_S158  -1.149739682  3.5483062 NA:072h      <NA> 072h B-072h-082_S158
B-072h-087_S163   2.474424568  3.3227377 NA:072h      <NA> 072h B-072h-087_S163
B-072h-105_S179  -4.870152140  4.2411364 NA:072h      <NA> 072h B-072h-105_S179
B-168h-010_S102   1.622330196 -2.3225392 NA:168h      <NA> 168h B-168h-010_S102
B-168h-020_S109   0.443072770 -1.9743801 NA:168h      <NA> 168h B-168h-020_S109
B-168h-021_S110   0.605650856 -1.8533296 NA:168h      <NA> 168h B-168h-021_S110
B-168h-034_S122   3.793121081 -0.4871453 NA:168h      <NA> 168h B-168h-034_S122
B-168h-036_S124   2.284190679 -1.0805896 NA:168h      <NA> 168h B-168h-036_S124
B-168h-068_S147   1.466100677  0.2844068 NA:168h      <NA> 168h B-168h-068_S147
B-168h-073_S151   1.542207973 -2.3339288 NA:168h      <NA> 168h B-168h-073_S151
B-168h-074_S152   3.269648176  0.1098486 NA:168h      <NA> 168h B-168h-074_S152
B-168h-110_S184  -4.812980315 -1.7470181 NA:168h      <NA> 168h B-168h-110_S184
C-000h-022_S111   0.421081990 -1.5410538 NA:000h      <NA> 000h C-000h-022_S111
C-000h-031_S120   1.924790839 -1.0966968 NA:000h      <NA> 000h C-000h-031_S120
C-000h-035_S123   5.767409891  5.6004148 NA:000h      <NA> 000h C-000h-035_S123
C-000h-049_S133   2.698040920 -2.0511850 NA:000h      <NA> 000h C-000h-049_S133
C-000h-072_S150   0.153482258 -1.8688691 NA:000h      <NA> 000h C-000h-072_S150
C-000h-085_S161   3.974074066 -1.5456674 NA:000h      <NA> 000h C-000h-085_S161
C-000h-089_S165   0.895956414 -4.6147697 NA:000h      <NA> 000h C-000h-089_S165
C-000h-099_S173   1.403521355 -1.2112024 NA:000h      <NA> 000h C-000h-099_S173
C-000h-101_S175   1.120086666 -1.3397290 NA:000h      <NA> 000h C-000h-101_S175
C-072h-015_S106   3.042787752  2.7031248 NA:072h      <NA> 072h C-072h-015_S106
C-072h-026_S115   2.986361551  1.2819011 NA:072h      <NA> 072h C-072h-026_S115
C-072h-053_S136   2.612581837 -3.0030911 NA:072h      <NA> 072h C-072h-053_S136
C-072h-065_S145   5.337826050 -1.4428190 NA:072h      <NA> 072h C-072h-065_S145
C-072h-096_S170   0.900925163  0.8642273 NA:072h      <NA> 072h C-072h-096_S170
C-072h-109_S183  -5.334425078 -2.4136414 NA:072h      <NA> 072h C-072h-109_S183
C-072h-118_S191  -7.231001196  3.2579731 NA:072h      <NA> 072h C-072h-118_S191
C-168h-001_S96    3.023570539 -0.7010362 NA:168h      <NA> 168h  C-168h-001_S96
C-168h-004_S98    1.823423831 -1.7113056 NA:168h      <NA> 168h  C-168h-004_S98
C-168h-007_S100   0.962411992 -2.2924658 NA:168h      <NA> 168h C-168h-007_S100
C-168h-017_S107   1.065361860  3.0621013 NA:168h      <NA> 168h C-168h-017_S107
C-168h-028_S117  -0.366006851 -2.9235285 NA:168h      <NA> 168h C-168h-028_S117
C-168h-059_S141   1.428278165 -1.9400322 NA:168h      <NA> 168h C-168h-059_S141
C-168h-078_S155   0.815843404  2.1067205 NA:168h      <NA> 168h C-168h-078_S155
C-168h-103_S177  -1.143954264  1.7431866 NA:168h      <NA> 168h C-168h-103_S177
C-168h-106_S180  -6.143535335 -2.2050061 NA:168h      <NA> 168h C-168h-106_S180
C-168h-115_S188 -10.687616920  0.8673982 NA:168h      <NA> 168h C-168h-115_S188
D-000h-002_S97    1.713323672 -1.8416271 NA:000h      <NA> 000h  D-000h-002_S97
D-000h-041_S128   3.324591638 -2.2369055 NA:000h      <NA> 000h D-000h-041_S128
D-000h-063_S143   2.509382117 -2.0362103 NA:000h      <NA> 000h D-000h-063_S143
D-000h-075_S153   2.748652742 -3.7119225 NA:000h      <NA> 000h D-000h-075_S153
D-000h-080_S156   2.282400058 -2.6198194 NA:000h      <NA> 000h D-000h-080_S156
D-000h-095_S169   2.144568051 -2.0766734 NA:000h      <NA> 000h D-000h-095_S169
D-000h-102_S176  -1.311142185 -2.3878073 NA:000h      <NA> 000h D-000h-102_S176
D-000h-107_S181  -5.927155889 -2.1570771 NA:000h      <NA> 000h D-000h-107_S181
D-000h-114_S187  -3.340403022 -0.8760670 NA:000h      <NA> 000h D-000h-114_S187
D-072h-008_S101   2.241312455 -1.5338208 NA:072h      <NA> 072h D-072h-008_S101
D-072h-019_S108   1.645271817 -0.5504861 NA:072h      <NA> 072h D-072h-019_S108
D-072h-032_S121   4.718969159 -1.1478291 NA:072h      <NA> 072h D-072h-032_S121
D-072h-038_S126   3.978578789 -1.2264232 NA:072h      <NA> 072h D-072h-038_S126
D-072h-040_S127   3.871264202 -0.6495841 NA:072h      <NA> 072h D-072h-040_S127
D-072h-091_S167   1.679631907  0.6100731 NA:072h      <NA> 072h D-072h-091_S167
D-072h-097_S171   1.934509572  4.2551949 NA:072h      <NA> 072h D-072h-097_S171
D-072h-100_S174  -2.205379764  3.1915375 NA:072h      <NA> 072h D-072h-100_S174
D-168h-025_S114   1.371362706 -1.7243487 NA:168h      <NA> 168h D-168h-025_S114
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