尝试使用 Python 焦点堆叠工具：IndexError：列表索引超出范围

问：

我正在尝试在我拥有的一组图像上使用此焦点堆叠工具，但我收到错误。这是我在 Linux 终端中使用的命令：

focusstack -i /projectnb/npbssmic/ac25/focus_stacking/Zstack -otest.png

我在虚拟环境中工作，我指定的目录是包含图像的目录的完整路径。它们是.tif格式。我将在下面放置此目录的屏幕截图，但首先这是我得到的错误：

INFO:focus_stack.focus_stack:reading images
INFO:focus_stack.focus_stack:aligning images
Traceback (most recent call last):
  File "/projectnb/npbssmic/ac25/.conda/envs/pytorch_env/bin/focusstack", line 33, in <module>
    sys.exit(load_entry_point('focus-stack', 'console_scripts', 'focusstack')())
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/projectnb/npbssmic/ac25/focus_stacking/focus-stack/focus-stack/focus_stack/run.py", line 58, in main
    stacked = stacker.focus_stack(image_files)
              ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/projectnb/npbssmic/ac25/focus_stacking/focus-stack/focus-stack/focus_stack/focus_stack.py", line 41, in focus_stack
    images = self._align_images(image_matrices)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/projectnb/npbssmic/ac25/focus_stacking/focus-stack/focus-stack/focus_stack/focus_stack.py", line 84, in _align_images
    aligned_imgs.append(images[0])
                        ~~~~~~^^^
IndexError: list index out of range

我查看了源代码，似乎这个错误是因为对我目录中的文件调用 cv2.imread（） 不起作用，但我不确定为什么。这是我所指的源代码：

def focus_stack(self, image_files: List[str]) -> np.ndarray:
        """Pipeline to focus stack a list of images."""
        image_matrices = self._read_images(image_files)
        images = self._align_images(image_matrices)
        laplacian = self._compute_laplacian(images)
        focus_stacked = self._find_focus_regions(images, laplacian)
        return focus_stacked

    @staticmethod
    def _read_images(image_files: List[str]) -> List[np.ndarray]:
        """Read the images into numpy arrays using OpenCV."""
        logger.info("reading images")
        return [cv2.imread(img) for img in image_files]

以下是目录的样子，以防万一：

目录